AUGUST 1999 


HIV isolated?

NATURE ALSO rejected this letter from Papadopulos-Eleopulos's team, which examined an article that Nature did publish. In "News and Views" (Nature Feb. 4), Robin Weiss and Richard Wrangham stated "the retrovirus that is the main cause of AIDS has been a puzzle ever since it was discovered by Barre-Sinoussi and her colleagues in 1983."


To isolate HIV, the authors used the long-accepted method for retroviral isolation: banding (ultracentrifugation) in density gradients ("Isolation of a T-Lymphotropic Retrovirus from a Patient at Risk for AIDS," Science 220, 1983). They claimed to have shown that the density of 1.16g/ml -- the sucrose density at which retroviral particles band -- contained "purified, labeled virus"; that is, the 1.16g/ml band contained nothing but retrovirus particles isolated from everything else.

Last year the French journalist Djamel Tahi interviewed the senior, most quoted author of the Barre-Sinoussi paper, Luc Montagnier of the Pasteur Institute (Continuum 5, 1998; www. virusmyth.com/aids/data/dtinterviewlm.htm). Tahi asked Montagnier why his group did not publish electron micrographs proving that the 1.16g/ml band contained isolated HIV particles. Montagnier answered: they published no such proof because, even after a "Roman effort," at the density of 1.16g/ml they could see no particles with "morphology typical of retroviruses." He gave similar answers to repeated questions, including "I repeat, we did not purify," that is, isolate HIV.

Given the important and highly significant differences in the consequences of these contradictory claims, it is a matter of urgency for the other 11 authors of the 1983 study, and especially Barre-Sinoussi and her co-workers from the Pasteur Institute, to either confirm or refute Montagnier's statements.

Eleni Papadopulos-Eleopulos (1)
Valendar F. Turner (2)
John M. Papadimitriou (3)
David Causer (Senior Physicist) (1)
Bruce Hedland-Thomas (1)
Barry Page (1)
Charles Geshekter (4)
Etienne DeHarven (5)
Helman Alphonso(6)
Todd Miller (7)

1) Dept of Medical Physics, Royal Perth Hospital, Western Australia.
2) Dept. of Emergency Medicine, Royal Perth Hospital, Western Australia;
3) Dept of Pathology, University of Western Australia;
4) Dept. of History California State University, Chico, California;
5) Professor (Emeritus) of Pathology, University of Toronto, Canada.
6) Head, Department of Research, Universidad Metropolitana Barranquilla, Colombia
7) Assistant Scientist,Department of Molecular and Cellular Pharmacology, University of Miami School of Medicine, Florida, USA

Eleni Papadopulos-Eleopulos, Biophysicist, Department of Medical Physics, Royal Perth Hospital, Wellington Street, Perth 6001, Western Australia.

Recent publications by the Papadopulos-Eleopulos group

ELENI PAPADOPULOS-ELEOPULOS'S team recently published the following articles, which -- like all their previous articles -- can be accessed at www.virusmyth.com/aids/perthgroup:

"A Critical Analysis of the Pharmacology of AZT and its Use in AIDS," (Current Medical Research and Opinion 15:3 Supplement 1, 1999).

"The Yin & Yang of HIV," (an extensive 2-part series in the alternative press magazine Nexus 6:4,5, Jun-Jul, Aug-Sep).

"Looking Back on the Oxidative Stress Theory of AIDS," (Continuum 5:5, midwinter 1998-99). This article provides an excellent synopsis of EPE's explanation for how all the risk factors (narcotics, anti-AIDS drugs, poverty, blood therapy, rectal semen) may cause both AIDS and "HIV."